Piccolo Antikörper (Atto 488)

Produktnummer ABIN2482389

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch Piccolo in WB und IHC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Piccolo Antikörper (Atto 488) (ABIN2482389)

Target: Piccolo (PIC)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Piccolo Antikörper ist konjugiert mit Atto 488

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Piccolo Antikörper (Atto 488)

Spezifität: Detects ~550 kDa. No cross-reactivity to Bassoon.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein A Purified

Immunogen: Recombinant Full length Protein, BC001304.1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:400)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2482389 was sufficient for detection of Piccolo in 10 μg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu Piccolo

Target: Piccolo (PIC)

Andere Bezeichnung: Piccolo

Hintergrund: Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).

Gen-ID: 27445

NCBI Accession: NP_055325

UniProt: Q9Y6V0